SLAS2016 Short Courses
Next Generation Sequencing Technology Fundamentals and Applications
Next generation sequencing (NGS) technologies allow laboratories to do genome-wide research that was previously only possible at large genome centers. This course teaches keys concepts and applications of major sequencing technologies (Illumina, 454, SOLiD, Ion Torrent & PacBio) including their technology basics, sample preps, data analysis and associated IT supports.
Who Should Attend
- Principal investigators
- Lab managers and technicians
- Biology oriented project scientists and postdocs
How You'll Benefit From This Course
- Understand the basic concepts and limitations of major NGS technologies
- Go through the workflows of NGS technology experiments and data analysis
- Learn major NGS applications including Genome Assembly, Target Sequencing, RNAseq, ChIPSeq, Single Cell Genomics
- NGS Technology Overview
- Error Rates and Limitations of NGS Technologies
- Experimental Workflows and Requirements
- Data Analysis Workflows and Requirements
- Information Technology Support
- Sample Prep Automation
Dr. Lin joined DAIT as a Senior Advisor for Bioinformatics in February 2013. Prior to coming to NIH, Dr. Lin was the founding Director of the Bioinformatics Core at the University of California Davis Genome Center for 7 years, and before that, he was in charge of the Computational Biology group of Southeast Collaboratory for Structural Genomics at University of Georgia for 4 years, and spent 5 years at the Brookhaven National Laboratory in New York where he played a key role in the modernization and operation of the Protein Data Bank (PDB). Dr. Lin received his Master of Science, Quantum Chemistry, at Jilin University, Changchun, China in 1993, and his Ph.D. in Computational Biology at Peking University, Beijing, China in 1996. Dr. Lin is widely recognized for his contributions to various “Big Data” initiatives and we anticipate that his expertise in complex data analysis, Bioinformatics, and high performance computational infrastructure planning will be assets to the Institute as he assumes responsibility for directing, and leading the bioinformatics activities within DAIT.
Dr. Lakdawalla joined Thermo Fisher Scientific in October 2014 to lead the development of sequencing applications for infectious diseases, reproductive genomics and for circulating DNA on the Ion Torrent sequencing platforms. Before Thermo Fisher, Abizar worked at Illumina for seven years with a focus on defining Illumina's next generation sequencing systems including the HiSeq and NextSeq platforms. At Illumina, Abizar worked with US and European scientific institutions to enable novel applications such as single cell analysis, epigenomics and protein interactions by next generation sequencing and has led multiple seminars and instructional courses on NGS and its applications. He received his B.Sc. in Microbiology and a Diploma in Medical Laboratory Technology from Bombay University, has, a M.Sc. in Biochemical Toxicology from the Bhabha Atomic Research Center in India and a PhD in Molecular Genetics from the Bowling Green State University, Ohio. After a post-doctorate in mouse transgenics from the Univ of California in Berkeley, Abizar transitioned to developing products for automated immunohistochemistry and in situ hybrization at BioGenex, California. He then moved to Applied Biosystems where he worked on microfluidic devices for sample prep and finally led a technology incubator focused on rapid commercialization of nascent technologies.